Sirt1 over-expression in retinal vascular endothelial cells does not protect against vascular degeneration in OIR.

<p><i>Tie2-Sirt1^OE</i> and littermate control mice were exposed to 75% oxygen from P7 to P12 to induce retinopathy. Retinas were dissected at P17 and stained with Isolectin B₄ to visualize vessels (red). a) Representative images of retina flat-mounts from <i>Tie2-Sirt1^OE</i> mice and littermate controls at P17 after OIR. Areas of vaso-obliteration(VO) or pathologic neovascularization (NV) were highlighted in white. Scale bar: 1000 µm. b) Quantification of retinal vaso-obliteration in OIR as percent of total retinal areas in <i>Tie2-Sirt1^OE</i> and littermate controls. c) Quantification of pathologic NV in OIR as percent of total retinal areas in <i>Tie2-Sirt1^OE</i> and littermate controls. n = 8–20/group; n.s.: not significant.