Western blot analysis of HSC 70 and DHAR expression patterns and the relative expressions on protein levels in <i>K. candel</i> leaves.

<p>(A) Antibodies against HSC 70 and DHAR were used to detect the change of protein levels in leaves in response to salt stress treatment of the plants. 50 µg protein samples were loaded in each lane, separated on 12% SDS-PAGE gel, followed by Western blotting and visualized with DAB as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083141#s5" target="_blank">Materials and Methods</a> section; (B) Gray analysis of the results by Quantity One software and the relative expression of HSC 70 and DHAR (spots 11 and 36) were shown. Bars represent the means of the relative intensity of the protein stains of three biological replicates of the control and salt-stressed treatments (150, 300, 450 and 600 mM NaCl).