Western blot analysis of protein expression of plasma membrane (PM) H⁺-ATPase and Na⁺/H⁺ antiporter (NHE-1).

<p>Shown in (A) and (C) are the relative protein expression level of <i>K. obovata</i> seedlings grown under 400 mM NaCl and various concentrations of SNP for 15 days. Shown in (B) and (D) represent the relative protein expression level of <i>K. obovata</i> seedlings treated with the following Hoagland solution-based solutions for 15 days: Control (only Hoagland solution), NaCl (400 mM), SNP (100 µM), NaCl+SNP (400 mM NaCl+100 µM SNP), cPTIO+L-NNA (200 µM cPTIO+100 µM L-NNA), NaCl+cPTIO+L-NNA (400 mM NaCl+200 µM cPTIO+100 µM L-NNA). Three independent experiments were performed. The relative intensity of bands (column charts) was quantified by densitometric analysis with Quantity One software and expressed relative to the intensity of the control band defined as 1. For each parameter, bars with different letters indicate significant difference at <i>P</i><0.05.