2D-DIGE quantitative phosphoproteomics analysis of GFPK7.

<p>An enlarged region of the 2D-DIGE gels showing Cy3-labeled WT stationary promastigotes and Cy5-labeled stationary GFPK7 promastigotes is presented. Spot ID 207 (white arrow) was over-represented in GFPK7. In the lower panel, a graphical representation of the BVA (Biological Variation Analysis) module of Decyder software (GE Healthcare) with statistics for spot ID 207 (2.97 fold and p = 0.00056). The spot was analyzed by mass spectrometry and identified as LmjF19.0160, a putative aminopeptidase.