Differential protein expression analysis of SW480-ADH Snail1 and Mock cells by 2D-DIGE electrophoresis.

<p>Nuclear extracts from SW480-ADH Mock and Snail1 cells were differentially labeled with Cy3 and Cy5 dyes. An internal standard combining proteins from both extracts was included in all gels after labeling with Cy2 dye. 3–10 NL IPG strips were used for the IEF and standard SDS-PAGE for the second dimension. Representative 2D image shows the spot map corresponding to the internal standard, which is common to all gels analyzed. Spots indicated with arrows were informative for protein identification by mass spectrometry (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010221#pone-0010221-t001" target="_blank">Table 1</a> for code assignment). Selected spots showed a statistical variance of volume ratios within the 95th confidence level (Student's <i>t</i>-test, <i>p</i><0.05).