Proliferation and cell activation at the different time frames.

<p>Cell activation at day 4 (A) and after four days after withdrawal of Tofa (B) is represented as mean fluorescence index (MFI) of CD25 ± SD of four independent experiments. Cell proliferation at day 4 (C) and after four days after withdrawal of Tofa (D) is represented as percentage of cells with reduced fluorescence of CFSE ± SD of four independent experiments. Statistical significance was calculated using one-way analysis of variance (ANOVA) and Bonferroni post-test. *** p<0.0001, **p<0.001.