Evaluation of T-cell proliferative dynamics from CFSE profiles.

<p>B10 recipient were treated as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004116#pone-0004116-g001" target="_blank">Fig. 1</a>. A) One representative example of the CFSE profiles observed at each time point (gated on CD8⁺ H7^a tetramer⁺ T cells) in the spleen and tumor. Numbers in the upper right corner of each panel represent the percentage of undivided cells (that is, cells showing the highest levels of CFSE). B) Mean (±SD) burst size, doubling time and expansion of H7^a-specific T cells between day 10 and day 15 in the spleen and tumor. The proliferative burst size and doubling time of anti-H7^a T cells were calculated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004116#s2" target="_blank">materials and methods</a>. Expansion corresponds to the mean number of cells on day 15/mean number of cells on day 10. Each group contained 3–5 mice. * <i>P</i><0.001 (spleen vs. tumor).