Reduced area of synaptic contacts in FAD mutant.

<p>(A and C) Example TE spines (red, transparent) with sites of synaptic contact (blue) for WT and FAD mutant conditions; scale bars = 200 nm. Two populations of vesicles are reconstructed, ‘docked’ (touching presynaptic membrane, orange) and ‘readily releasable’ (within one vesicle diameter of presynaptic active zone, green). (B and D) Each synaptic site viewed head-on for WT and FAD mutant conditions; scale bars = 200 nm. (E) Cumulative distribution plot of synaptic contact site area. (F) Number of vesicles per unit area of synaptic contact for ‘docked’ and ‘readily-releasable’ vesicle pools (synaptic contacts: n = 22 for each condition). (G and H) Number of vesicles plotted against synaptic contact area for each condition for ‘docked’ (G) and ‘readily-releasable’ (H) pools.