<p>(A) Tet-on-eGFP TG piglets. (B) PCR was performed using genomic DNA isolated from various organs and tissues of SCNT piglets and primers designed to amplify eGFP. GAPDH and CaBP 9k were amplified as controls. eGFP was amplified from all the tissues and organs tested. N, negative control; P, positive control.</p
<p>Detection of tissue distribution of P1 by PCR in infected and control pigs.</p
<p>Southern blot was performed under optimized condition with a Dig-lableled probe of 649 bp fragmen...
<p>Gene expression resulted from Agilent microarray and real-time PCR between 8 experimental F2 and ...
<p>A: <i>In vivo</i> fluorescence in founder pigs. B: Screen of transgenic founder pigs by PCR. M: D...
<p>Two piglets were sacrificed at 30-days-old and 50-days to analyze eGFP expression in various orga...
<p>(A) eGFP was not expressed in the hoop of TG piglet before doxycycline treatment. eGFP was expres...
(A) Genomic PCR analysis of the F0-generation to identify TG pigs. P1, P2, P4, P5, P6, P8, P11 and P...
Site-specific nucleases (ZFN, Tal effector nucleases and CRISPRs) boosted the genome editing of diff...
<p>(A) Absolute quantitative real-time PCR analysis of GFP copy number from newborn to maturity in f...
<p>(<b>A</b>) PCR analysis of genomic DNA of piglet DNA was shown. Genomic DNA of wild-type, 134-fet...
<p>(A) Two cloned fetuses (named 1## and 2##) derived from 17# transgenic clones by SCNT at 15 days ...
<p>(A) Cloned piglets produced from apoE-shRNA1 transfected fibroblast cells. (B) Epifluorescence im...
<p>(A) The fusion rate, cleavage rate, percentage of embryos that developed to blastocyst stage, and...
<p>Agarose gel electrophoresis of PCR products shows expression of EGF (lane one), TGFβ1 (lane two),...
The objective of this experiment was to identify the novel SNP on porcine FTH genes. Moreover, their...
<p>Detection of tissue distribution of P1 by PCR in infected and control pigs.</p
<p>Southern blot was performed under optimized condition with a Dig-lableled probe of 649 bp fragmen...
<p>Gene expression resulted from Agilent microarray and real-time PCR between 8 experimental F2 and ...
<p>A: <i>In vivo</i> fluorescence in founder pigs. B: Screen of transgenic founder pigs by PCR. M: D...
<p>Two piglets were sacrificed at 30-days-old and 50-days to analyze eGFP expression in various orga...
<p>(A) eGFP was not expressed in the hoop of TG piglet before doxycycline treatment. eGFP was expres...
(A) Genomic PCR analysis of the F0-generation to identify TG pigs. P1, P2, P4, P5, P6, P8, P11 and P...
Site-specific nucleases (ZFN, Tal effector nucleases and CRISPRs) boosted the genome editing of diff...
<p>(A) Absolute quantitative real-time PCR analysis of GFP copy number from newborn to maturity in f...
<p>(<b>A</b>) PCR analysis of genomic DNA of piglet DNA was shown. Genomic DNA of wild-type, 134-fet...
<p>(A) Two cloned fetuses (named 1## and 2##) derived from 17# transgenic clones by SCNT at 15 days ...
<p>(A) Cloned piglets produced from apoE-shRNA1 transfected fibroblast cells. (B) Epifluorescence im...
<p>(A) The fusion rate, cleavage rate, percentage of embryos that developed to blastocyst stage, and...
<p>Agarose gel electrophoresis of PCR products shows expression of EGF (lane one), TGFβ1 (lane two),...
The objective of this experiment was to identify the novel SNP on porcine FTH genes. Moreover, their...
<p>Detection of tissue distribution of P1 by PCR in infected and control pigs.</p
<p>Southern blot was performed under optimized condition with a Dig-lableled probe of 649 bp fragmen...
<p>Gene expression resulted from Agilent microarray and real-time PCR between 8 experimental F2 and ...