<p>(A) Two cloned fetuses (named 1## and 2##) derived from 17# transgenic clones by SCNT at 15 days after transplantation. (B) Genomic PCR analysis of fetuses 1## and 2## stably transfected with pROP2-EGFP. NC: negative control. (C) EGFP expressed in genital ridges isolated from 2## fetus but not in other tissues, such as intestinal tissues. Scale bars = 1 mm. (D) EGFP reactivated in morulae and blastocysts after the second SCNT was performed using Oct4-EGFP transgenic fibroblasts isolated from fetus 1## and 2## as donors. Parthenogenetic blastocysts did not express EGFP. Scale bars = 100 µm.</p
The development of somatic cell nuclear transfer (SCNT) embryos critically depends on appropriate re...
<p>A,D) Blastocysts produced by injection with (ME)<i>egfp</i>:Tn5 transposome. B,E) Blastocysts pro...
<p>(<b>A</b>) ES cells were transduced with NT or OT and picked ES cell clones carrying one integran...
<p>(A) The fusion rate, cleavage rate, percentage of embryos that developed to blastocyst stage, and...
<p>(A) PCR genotyping detected the KI fetuses by using primers indicated in the diagram. PCR reveale...
<p>(A) SCNT embryos generated using somatic cells derived from a TG piglet were cultured in the abse...
<p>(A) Tet-on-eGFP TG piglets. (B) PCR was performed using genomic DNA isolated from various organs ...
<p>Western blotting analysis to detect eGFP protein in 1: transgenic eGFP fetal chorion; 2: transgen...
<p>(A) SCNT was performed using eGFP-positive pFF. The resulting embryos were cultured in the presen...
(A) In vitro and in vivo development of SCNT embryos injected with extra-NPBs (SCNT+NPB). The rates ...
<p>Two piglets were sacrificed at 30-days-old and 50-days to analyze eGFP expression in various orga...
(A) NPB volume and single NPB formation in extra-NPB-injected SCNT embryos (SCNT+NPB). The total vol...
<p>A, Igf2 transcripts in fetuses, and B, Igf2 expression levels in piglets. IV, the in vivo produce...
<p>Fold change analysis with a cutoff of f >2 identified the number of differentially expressed gene...
<p>(a) PCR analysis of single blastocyst to verify excision of the selectable marker gene in the exc...
The development of somatic cell nuclear transfer (SCNT) embryos critically depends on appropriate re...
<p>A,D) Blastocysts produced by injection with (ME)<i>egfp</i>:Tn5 transposome. B,E) Blastocysts pro...
<p>(<b>A</b>) ES cells were transduced with NT or OT and picked ES cell clones carrying one integran...
<p>(A) The fusion rate, cleavage rate, percentage of embryos that developed to blastocyst stage, and...
<p>(A) PCR genotyping detected the KI fetuses by using primers indicated in the diagram. PCR reveale...
<p>(A) SCNT embryos generated using somatic cells derived from a TG piglet were cultured in the abse...
<p>(A) Tet-on-eGFP TG piglets. (B) PCR was performed using genomic DNA isolated from various organs ...
<p>Western blotting analysis to detect eGFP protein in 1: transgenic eGFP fetal chorion; 2: transgen...
<p>(A) SCNT was performed using eGFP-positive pFF. The resulting embryos were cultured in the presen...
(A) In vitro and in vivo development of SCNT embryos injected with extra-NPBs (SCNT+NPB). The rates ...
<p>Two piglets were sacrificed at 30-days-old and 50-days to analyze eGFP expression in various orga...
(A) NPB volume and single NPB formation in extra-NPB-injected SCNT embryos (SCNT+NPB). The total vol...
<p>A, Igf2 transcripts in fetuses, and B, Igf2 expression levels in piglets. IV, the in vivo produce...
<p>Fold change analysis with a cutoff of f >2 identified the number of differentially expressed gene...
<p>(a) PCR analysis of single blastocyst to verify excision of the selectable marker gene in the exc...
The development of somatic cell nuclear transfer (SCNT) embryos critically depends on appropriate re...
<p>A,D) Blastocysts produced by injection with (ME)<i>egfp</i>:Tn5 transposome. B,E) Blastocysts pro...
<p>(<b>A</b>) ES cells were transduced with NT or OT and picked ES cell clones carrying one integran...