Suppression of autologous PBMCs by CMV-stimulated CD25+ cells isolated following CMVpp65 stimulation.

<p>Suppressive capacity was assessed in CD25-positive and CD25-negative fractions after magnetic selection following CMVpp65 stimulation between G-CSF-mobilized (A) and non-mobilized (B) donors. CFSE-labelled PBMCs were cultured at two ratios in the presence of SEB for 5 days. Cumulative data in G-CSF-mobilized (<i>n</i> = 5) and non-mobilized (<i>n</i> = 5) donors are shown at a ratio of 1∶1 (diagonal bars) and 1∶2 (shaded bars) PBMCs to CD25+ selected T cells after calculating percentage of suppression as indicated in the Material and Methods section. Data are presented as mean values with standard deviation (C). Unlabelled, PBMCs alone (CFSE) and PBMCs with SEB were cultured as experimental controls. **<i>p</i><0.01, in an unpaired <i>t</i> test.