Phylogenetic tree highlighting the position of strain AK16^T.

<p>Phylogenetic tree highlighting the position of strain AK16^T relative to other related type strains within the family <i>Vibrionaceae</i>. Strains shown are those within the <i>Vibrionaceae</i> having corresponding NCBI genome project ids listed within. The tree uses sequences aligned by the Clustal W aligner. The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura-Nei model <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085590#pone.0085590-Tamura1" target="_blank">[24]</a>. The tree with the highest log likelihood (−5929.7876) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically as follows. When the number of common sites was <100 or less than one fourth of the total number of sites, the maximum parsimony method was used; otherwise BIONJ method with MCL distance matrix was used. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 36 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 1249 positions in the final dataset. Evolutionary analyses were conducted in MEGA5 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085590#pone.0085590-Tamura2" target="_blank">[25]</a>. <i>Gallaecimonas pentaromativorans</i> CEE 131^T (FM955224) was used as an outgroup.