Postconditioning activates Akt and eNOS.

<p><b>A.</b> Western blot of total eNOS and GADPH in WT (C57BL6/J), S1176D mice (S1176ki), and eNOS ko mice. <b>B.</b> Western blot demonstrating phosphorylation (Ser1176) and total protein levels of eNOS in wild-type mice under conditions of control (CTL), MIR, and MIPc. <b>C.</b> Representative Western blot demonstrating phosphorylated (Ser473) and total protein levels of Akt. Densities (arbitrary units, AU) show that MIPc phosphorylates Akt in WT and eNOS ko mice. n = 5 per group. Data are expressed as the mean±SD. *P<0.05.