Experimental protocol.

<p>(1) ND-sh (n = 24), placing the ligature under LCA; (2) ND-IR (n = 30), occlusion of LCA for 30 min, was followed by 3 h of reperfusion; (3) ND-RIPostC (n = 30), three RIPostC cycles were applied at the onset of coronary reperfusion period; (4) ND-IR+RIPostC +3MA (n = 30), three RIPostC cycles were applied at the onset of coronary reperfusion period, and received 3MA treatment 10 min before coronary reperfusion; (5) DM-sh (n = 24), placing the ligature under LCA; (6) DM-IR (n = 30), occlusion of LCA for 30 min, was followed by 3 h of reperfusion; (7) DM-RIPostC (n = 30), three RIPostC cycles were applied at the onset of coronary reperfusion period; (8) ND-3MA (n = 30), three RIPostC cycles were applied at the onset of coronary reperfusion period, and received 3-MA treatment 10 min before coronary reperfusion.