Experiment protocol.

<p>Group A: RIPoC+IPoC group, received the combined algorithms of RIPoC and IPoC; Group B: RIPoC group, which received 3 cycles of 30 seconds reperfusion and re-occlusion on the limb at the onset of reperfusion; Group C: IPoC group, received 3 cycles of 30 seconds reperfusion and re-occlusion on the LAD; Group D: IR group, received reperfusion without interruption after ischemia; Group E: sham group, received only open chest; Group F: NAC+IR group, ROS scavenger N-acetylcyseine was administered in IR group. Group A-E underwent ischemic heart detections including on anti-oxidative stress index, mitochondrial function, inflammation reaction, stem cell survival and homing. Group A-F underwent MSCs intramyocardial transplantation and cardiac function tests. MSCs: mesenchymal stem cells. SDF-1α: stromal cell derived factor-1 alpha. VEGF: vascular endothelium growth factor. RISK: reperfusion injury survival kinase. NAC: N-acetylcyseine.