Gene expression profiles of whole transcriptome microarrays.

<p>A. Two-way hierarchical clustering heatmap of differentially expressed genes between HIC-1 activated MCF-7 cells and control. Gene expression was significantly different between the two groups. B. Bar chart of up- and downregulated genes based on HIC-1 reactivation in MCF-7 cancer cells. C. mRNA verification of 10 selected genes by quantitative RT-PCR on the same six samples used in the microarray study. Five genes were upregulated (TIMP3, NTN4, BIK, CASP4, and IFI35), and five were downregulated (SKA3, HMMR, CENPF, CKS1B, and UBE2C), which is consistent with the results of the microarray assay.