Diet-mediated effects on fecal microflora.

<p>(A) DGGE fingerprints of fecal DNA from the PD-fed (lanes 1–4) and TD-fed mice (lanes 5–8) (n = 4 mice/group). Data shown is one representative of three independent experiments. (B) Cluster analysis showing the similarity measurements of the DGGE banding patterns shown in panel A. (C) Metagenomic analysis showing the abundance of bacterial phyla present in feces from PD- and TD-fed mice (n = 2 mice/group). (D) Abundance of bacterial families in Firmicutes phylum present in feces from PD- and TD-fed mice (n = 2 mice/group). Significant difference was found in multiple bacterial phyla and families between PD and TD (***P<0.0001, Pearson Chi-Square Goodness of Fit test). DGGE, denaturing gradient gel electrophoresis; PD, purified diet; TD, Teklad diet.