Localization of STAT5A and STAT5B, and monomers and dimers of STAT5A and STAT5B.

<p><b>A</b> demonstrates translocation of STAT5A and STAT5B into cell nuclei after 30-2 (40× confocal). Yellow, STAT5A; purple, STAT5B; blue, nucleus. <b>B</b> and <b>C</b>. Detection of STAT5A and STAT5B proteins in cytoplasmic or nuclear proteins fractionated from CD4⁺ T cells after PHA-P stimulation for 3 days followed by incubation with rhIL-2 for 30 min. <b>B</b>. STAT5A monomer (91 kDa, arrow 1) and STAT5A dimer (arrow 2) in native cytoplasmic or nuclear proteins, detected with anti-STAT5A Ab. <b>C</b>. STAT5B monomer (90 kDa, arrow 3) and STAT5B dimer (arrow 4) in native cytoplasmic or nuclear proteins, detected using anti-STAT5B Ab. <b>D</b>. Detection of phosphorylated STAT5 proteins in STAT5A- or STAT5B- immunoprecipitated nuclear proteins fractionated from CD4⁺ T cells after PHA-P stimulation for 3 days followed by incubation with rh-IL-2 for 0 min, 30 min or 3 days. <b>E</b>. Detection of phosphorylated STAT5 proteins in cytoplasmic or nuclear proteins fractionated from CD4⁺ T cells after PHA-P stimulation for 3 days followed by incubation with rhIL-2 for 3 days, and control (unstimulated condition).