STAT5b inhibits STAT1 and STAT3 activation in TL-1 cells.

<p>STAT1 (A, B), STAT3 (C, D), and STAT5b (E, F) were knocked down using siRNA in IL-2 supplemented TL-1 cells. Protein lysates were harvested 48 h after siRNA transfection. Each blot was probed for p-STAT1, STAT1, p-STAT3, STAT3, p-STAT5, STAT5, and β actin. p-STAT proteins were normalized to their respective total STAT proteins. STAT1, STAT3, and STAT5 were normalized to β actin. Representative western blots are shown (A, C, E) as well as quantification of experimental replicates (B, D, F). Student’s T test was used to determine significance compared to scrambled siRNA. * = p<0.05, ** = p<0.01, *** = p<0.005, **** = p<0.001. Data are presented as mean +/- Stdev (n = 3 biological replicates).