There was no association found between the level of HMGB-1 and neovascularization in the retina.

<p>Neovascularization was induced by subretinally injecting Matrigel either with PBS (<b>A</b>) or mixed with HMGB-1 (<b>B</b>). Neovascularization was allowed to develop for 10 days. Blood vessels were labeled with DiI and visualized by fluorescence microscopy (white arrows indicate neovascularization in the Matrigel injected area. Ch, choroids; M, Matrigel, R retina. Scale bar: 200 µm). (<b>C</b>) Quantitative analysis shows no difference in the levels of neovascularization between HMGB1-treated eyes compared to control eyes. (<b>D</b>) <i>VEGF-A</i> expression was not changed in glial cells (astrocytea and microglia) treated with HMGB-1 and PBS (control). (<b>E</b>) Examination by western blot of vitreous humor from both hyperoxia-injured and room air control animals (obtained from postnatal days 15 and 17 pups) did not show a statistically significant difference between them. Results of analysis of western blots (top) are expressed as a percentage of corresponding value in the control eyes ± SEM (n = 6).