Comparative efficiency of MS2 and AcNPV viral simulants recovery.

<p>Recovery and extraction efficiencies were assessed by qPCR. Tris-Mg-Ca buffer (A and B) is used to recover viruses spiked in different matrices and to extract nucleic acids using different extraction kits, as indicated. One-tailed Wilcoxon test was used to assess if extraction efficiency was significantly lower with NucliSens and Invisorb as compared to EZ1 extraction kit (*p = 0.05). Mean percentage and SD (n = 3) of MS2 RNA (A) and AcNPV DNA extraction efficiency (B) from a liquid, sand or soil matrix after data normalization with the EZ1 extraction kit. (C and D) virus recovery and extraction from a soil matrix using NucliSens extraction kit, and assessing the impact of different types of recovery buffers, as indicated (one-tailed Wilcoxon, *p = 0.05). (C) Mean percentage and SD (n = 3) of MS2 RNA extraction comparing the effect of glycine and Tris-Mg-Ca recovery buffers. (D) Mean percentage and SD (n = 3) of AcNPV DNA extraction: for virus recovery, glycine buffer alone was compared to glycine buffer supplemented with BSA, NaCl or both.