Total virus particle recovery as a function (average SEM) of the concentration diafiltration volume for (A) different R&D prototypes (B) pilot production devices.

Economic pseudo-affinity purification of mammalian cell culture-derived influenza virus particles by sulphated cellulose membranes

Opitz, L.
Solf, N.
Reichl, U.
Wolff, M. W.

Influenza is a contagious respiratory illness that can cause mild to severe symptoms and can lead to...

Sulphated cellulose membranes – an economic virus capturing method for influenza virus purification

Opitz, L.
Solf, N.
Reichl, U.
Wolff, M. W.

Influenza is one of the most worldwide spread diseases. Besides antiviral medical treatments, prophy...

Printed in Great Britain A Comparison of Four Methods Used to Concentrate Rous Sarcoma Virus from Tissue Culture Fluids

D. L. Bronson
A. Y. Ell Iott*t
Donna R Itz I T
Accepted Ju Ly I

August 2015

Three methods of pelleting, pelleting followed by Pronase treatment, polyethylene glycol (PEG)-Prona...

DNA clearance as a function (average SEM) of the concentration/diafiltration volume for different R&D prototypes (Fig.A) and for the pilot production devices (Fig.B).

Piergiuseppe Nestola (2218627)
Duarte L. Martins (1628107)
Cristina Peixoto (530608)
Susanne Roederstein (2218630)
Tobias Schleuss (2218633)
Paula M. Alves (139464)
José P. B. Mota (2218636)
Manuel J. T. Carrondo (182731)

December 2014

The plots display the same process first operating in concentration mode and then diafiltration. ...

Recovery of infectious particles (IP) and processing time for 10-fold concentration for the different pilot production and R&D UF devices.

Piergiuseppe Nestola (2218627)
Duarte L. Martins (1628107)
Cristina Peixoto (530608)
Susanne Roederstein (2218630)
Tobias Schleuss (2218633)
Paula M. Alves (139464)
José P. B. Mota (2218636)
Manuel J. T. Carrondo (182731)

December 2014

Recovery of infectious particles (IP) and processing time for 10-fold concentration for the diffe...

Viral recovery rate of collection and quantification system.

Etsuko Hatagishi (607423)
Michiko Okamoto (461195)
Suguru Ohmiya (607424)
Hisakazu Yano (319302)
Toru Hori (607425)
Wakana Saito (607426)
Hiroshi Miki (607427)
Yasushi Suzuki (155330)
Reiko Saito (155351)
Taro Yamamoto (123751)
Makoto Shoji (607428)
Yoshihisa Morisaki (607429)
Soichiro Sakata (490363)
Hidekazu Nishimura (490362)

August 2014

aThe experiments were performed in triplicate for the high amount experiment controls and the num...

Evaluation of novel large cut-off ultrafiltration membranes for adenovirus serotype 5 (Ad5) concentration.

Piergiuseppe Nestola
Duarte L Martins
Cristina Peixoto
Susanne Roederstein
Tobias Schleuss
Paula M Alves
José P B Mota
Manuel J T Carrondo

The purification of virus particles and viral vectors for vaccine and gene therapy applications is g...

Data from: Evaluation of novel large cut-off ultrafiltration membranes for adenovirus serotype 5 (Ad5) concentration.

Nestola, Piergiuseppe
Martins, Duarte Lima
Peixoto, Cristina
Roederstein, Susanne
Schleuss, Tobias
Alves, Paula Maria
Mota, José Paulo B.
Carrondo, Manuel J. T.

January 2015

The purification of virus particles and viral vectors for vaccine and gene therapy applications is g...

Recovery efficiencies of nucleic acid extraction kits as measured by quantitative LightCycler (TM) PCR

Aims-To compare the efficiency of five nucleic acid extraction kits for processing clinical material...

Comparative efficiency of MS2 and AcNPV viral simulants recovery.

Mostafa Bentahir (520437)
Frederic Laduron (520438)
Leonid Irenge (396988)
Jérôme Ambroise (3498626)
Jean-Luc Gala (396991)

February 2014

Recovery and extraction efficiencies were assessed by qPCR. Tris-Mg-Ca buffer (A and B) is used t...

Trade-off between throughput, indicated as liter of feed processed in the unit of time (h), given a defined membrane area (m2), and infective particle recovery yield.

Piergiuseppe Nestola (2218627)
Duarte L. Martins (1628107)
Cristina Peixoto (530608)
Susanne Roederstein (2218630)
Tobias Schleuss (2218633)
Paula M. Alves (139464)
José P. B. Mota (2218636)
Manuel J. T. Carrondo (182731)

December 2014

The values referrer at 10 times concentration factor. The orange area on the right top corner dep...

Elution and concentration of viruses from sediments

Kata Farkas
James E McDonald
Shelagh K Malham
Davey L Jones

December 2018

This benchtop protocol describes the recovery of viruses from sediment using a beef extract-based el...

Pseudo-affinity capture of cell culture derived influenza virus particles by sulphated cellulose membranes : an economic tool for vaccine production

Opitz, L.
Solf, N.
Reichl, U.
Wolff, M.

Influenza is a contagious respiratory illness that can cause mild to severe symptoms and can lead to...

Development of a viral concentration method for bottled water stored in hydrophobic support

Gassilloud, B.
Huguet, L.
Maul, A.
Gantzer, C.

June 2007

International audienceSeveral protocols have been described for the detection of genomes of enteric ...

A Combined Ultrafiltration/Diafiltration Process for the Purification of Oncolytic Measles Virus

Daniel Loewe
Hauke Dieken
Tanja A. Grein
Denise Salzig
Peter Czermak

January 2022

Measles virus (MV) is an important representative of a new class of cancer therapeutics known as onc...

Economic pseudo-affinity purification of mammalian cell culture-derived influenza virus particles by sulphated cellulose membranes

Opitz, L.
Solf, N.
Reichl, U.
Wolff, M. W.

Influenza is a contagious respiratory illness that can cause mild to severe symptoms and can lead to...

Sulphated cellulose membranes – an economic virus capturing method for influenza virus purification

Opitz, L.
Solf, N.
Reichl, U.
Wolff, M. W.

Influenza is one of the most worldwide spread diseases. Besides antiviral medical treatments, prophy...

Printed in Great Britain A Comparison of Four Methods Used to Concentrate Rous Sarcoma Virus from Tissue Culture Fluids

D. L. Bronson
A. Y. Ell Iott*t
Donna R Itz I T
Accepted Ju Ly I

August 2015

Three methods of pelleting, pelleting followed by Pronase treatment, polyethylene glycol (PEG)-Prona...

DNA clearance as a function (average SEM) of the concentration/diafiltration volume for different R&D prototypes (Fig.A) and for the pilot production devices (Fig.B).

Piergiuseppe Nestola (2218627)
Duarte L. Martins (1628107)
Cristina Peixoto (530608)
Susanne Roederstein (2218630)
Tobias Schleuss (2218633)
Paula M. Alves (139464)
José P. B. Mota (2218636)
Manuel J. T. Carrondo (182731)

December 2014

The plots display the same process first operating in concentration mode and then diafiltration. ...

Recovery of infectious particles (IP) and processing time for 10-fold concentration for the different pilot production and R&D UF devices.

Piergiuseppe Nestola (2218627)
Duarte L. Martins (1628107)
Cristina Peixoto (530608)
Susanne Roederstein (2218630)
Tobias Schleuss (2218633)
Paula M. Alves (139464)
José P. B. Mota (2218636)
Manuel J. T. Carrondo (182731)

December 2014

Recovery of infectious particles (IP) and processing time for 10-fold concentration for the diffe...

Viral recovery rate of collection and quantification system.

Etsuko Hatagishi (607423)
Michiko Okamoto (461195)
Suguru Ohmiya (607424)
Hisakazu Yano (319302)
Toru Hori (607425)
Wakana Saito (607426)
Hiroshi Miki (607427)
Yasushi Suzuki (155330)
Reiko Saito (155351)
Taro Yamamoto (123751)
Makoto Shoji (607428)
Yoshihisa Morisaki (607429)
Soichiro Sakata (490363)
Hidekazu Nishimura (490362)

August 2014

aThe experiments were performed in triplicate for the high amount experiment controls and the num...

Evaluation of novel large cut-off ultrafiltration membranes for adenovirus serotype 5 (Ad5) concentration.

Piergiuseppe Nestola
Duarte L Martins
Cristina Peixoto
Susanne Roederstein
Tobias Schleuss
Paula M Alves
José P B Mota
Manuel J T Carrondo

The purification of virus particles and viral vectors for vaccine and gene therapy applications is g...

Data from: Evaluation of novel large cut-off ultrafiltration membranes for adenovirus serotype 5 (Ad5) concentration.

Nestola, Piergiuseppe
Martins, Duarte Lima
Peixoto, Cristina
Roederstein, Susanne
Schleuss, Tobias
Alves, Paula Maria
Mota, José Paulo B.
Carrondo, Manuel J. T.

January 2015

The purification of virus particles and viral vectors for vaccine and gene therapy applications is g...

Recovery efficiencies of nucleic acid extraction kits as measured by quantitative LightCycler (TM) PCR

Aims-To compare the efficiency of five nucleic acid extraction kits for processing clinical material...

Comparative efficiency of MS2 and AcNPV viral simulants recovery.

Mostafa Bentahir (520437)
Frederic Laduron (520438)
Leonid Irenge (396988)
Jérôme Ambroise (3498626)
Jean-Luc Gala (396991)

February 2014

Recovery and extraction efficiencies were assessed by qPCR. Tris-Mg-Ca buffer (A and B) is used t...

Trade-off between throughput, indicated as liter of feed processed in the unit of time (h), given a defined membrane area (m2), and infective particle recovery yield.

Piergiuseppe Nestola (2218627)
Duarte L. Martins (1628107)
Cristina Peixoto (530608)
Susanne Roederstein (2218630)
Tobias Schleuss (2218633)
Paula M. Alves (139464)
José P. B. Mota (2218636)
Manuel J. T. Carrondo (182731)

December 2014

The values referrer at 10 times concentration factor. The orange area on the right top corner dep...

Elution and concentration of viruses from sediments

Kata Farkas
James E McDonald
Shelagh K Malham
Davey L Jones

December 2018

This benchtop protocol describes the recovery of viruses from sediment using a beef extract-based el...

Pseudo-affinity capture of cell culture derived influenza virus particles by sulphated cellulose membranes : an economic tool for vaccine production

Opitz, L.
Solf, N.
Reichl, U.
Wolff, M.

Influenza is a contagious respiratory illness that can cause mild to severe symptoms and can lead to...

Development of a viral concentration method for bottled water stored in hydrophobic support

Gassilloud, B.
Huguet, L.
Maul, A.
Gantzer, C.

June 2007

International audienceSeveral protocols have been described for the detection of genomes of enteric ...

A Combined Ultrafiltration/Diafiltration Process for the Purification of Oncolytic Measles Virus

Daniel Loewe
Hauke Dieken
Tanja A. Grein
Denise Salzig
Peter Czermak

January 2022

Measles virus (MV) is an important representative of a new class of cancer therapeutics known as onc...

Economic pseudo-affinity purification of mammalian cell culture-derived influenza virus particles by sulphated cellulose membranes

Opitz, L.
Solf, N.
Reichl, U.
Wolff, M. W.

Influenza is a contagious respiratory illness that can cause mild to severe symptoms and can lead to...

Sulphated cellulose membranes – an economic virus capturing method for influenza virus purification

Opitz, L.
Solf, N.
Reichl, U.
Wolff, M. W.

Influenza is one of the most worldwide spread diseases. Besides antiviral medical treatments, prophy...

Printed in Great Britain A Comparison of Four Methods Used to Concentrate Rous Sarcoma Virus from Tissue Culture Fluids

D. L. Bronson
A. Y. Ell Iott*t
Donna R Itz I T
Accepted Ju Ly I

August 2015

Three methods of pelleting, pelleting followed by Pronase treatment, polyethylene glycol (PEG)-Prona...

Total virus particle recovery as a function (average SEM) of the concentration diafiltration volume for (A) different R&D prototypes (B) pilot production devices.

Abstract

Extracted data

Total virus particle recovery as a function (average SEM) of the concentration diafiltration volume for (A) different R&D prototypes (B) pilot production devices.

Abstract

Extracted data

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