Phosphorylation levels of K_V4.3 and K_V2.2 in normal, sham, and obstruction groups.

<p>Phosphorylation of Kv4.3 and Kv2.2 were examined by immunoprecipitation (IP) with ant-Kv4.3 antibody followed by IB with antibody against p-threonine, p-serine and Kv4.3 or IP with anti- Kv2.2 antibody followed by IB with antibody against p-threonine, p-serine and Kv2.2 (A). Corresponding bands were scanned and the phosphorylation band optical density was normalized by the total protein density. Data were the means ± SD and were expressed as folds versus normal. *<i>P</i><0.05 versus normal and sham, n = 5 (B).