The protein levels of P38, p-P38, ERK1/2, p-ERK1/2, AKT and p-AKT and the ratio of phosphorylated proteins in the corresponding proteins.

<p>(a-c) The protein levels of P38, p-P38, ERK1/2, p-ERK1/2, AKT and p-AKT are shown. α-Tubulin was used as the loading control. The molecular masses of P38 or p-P38, ERK1/2 or p-ERK1/2 and AKT or p-AKT were approximately 38 kDa, 42/44 kDa, 56 kDa, respectively. (A-C) The protein expression levels of these proteins were then analyzed by Image J software. Graphical representation demonstrating the protein expression and phosphorylation levels between the dorsal skin samples of the three groups obtained from western blotting assays. (D) The ratio of phosphorylated proteins is shown as 1 in WT mice. In the above figures, the results of one representative experiment of three independent experiments are presented. Each bar represents the mean ± SEM (n = 3). Different superscripts on the bar indicate a statistically significant difference (P < 0.01).