TsV and Ts1 stimulation increases the mRNA expression of <i>Tlr2, Cd14, Myd88</i> and <i>Ptgs2</i> in peritoneal macrophages.

<p>Adherent macrophages from C57Bl/6 (WT) mice were treated with TsV or Ts1 (50 µg/ml) for 4 h. Unstimulated macrophages were used as the negative control. The cells were lysed, and total RNA was extracted. qRT-PCR was performed to determine the relative expression levels of transcripts encoding lipid metabolism enzymes, TLRs and adaptor proteins. The results were normalized to the expression levels of the endogenous internal controls <i>Actb</i>, <i>Gapdh</i> and <i>Tbp</i>. The 2^–ΔΔCt method was used for the analysis of the qRT-PCR data. *<i>p</i><0.05 (one-way ANOVA followed by Dunnett’s post-test) compared to medium alone. Statistically significant changes were considered when <i>p</i><0.05 and any gene presented a fold-change >2.0. The results are presented as the fold-change measured from 2 independent experiments.