Quantitative RT-PCR confirms changes in gene expression.

<p>a. The ΔC_T value is determined by subtracting the average Actin C_T value from the average Gene C_T value. The standard deviation of the difference is calculated from the standard deviations of the gene and actin values using the Comparative Method.</p><p>b. The calculation of ΔΔC_T involves subtraction by the ΔC_T calibrator value. This is subtraction of an arbitrary constant, so the standard deviation of ΔΔC_T is the same as the standard deviation of the ΔC_T value.</p><p>c. The range given for gene relative to WT/MM is determined by evaluating the expression: 2∧ –ΔΔC_T with ΔΔC_T + s and ΔΔC_T – s, where s = the standard deviation of the ΔΔC_T value.