Pd allergy is induced by hapten-specific responseand transferable through pathogenic T cell transfer.

<p>(A) Ear thickness was measured before challenge and at the indicated time point after challenge. CHS reaction was elicited in Pd plus LPS-sensitized mice (left panel) or DNFB-sensitized mice (right panel), as described. As negative controls (open circles), mice were i.p. injected with PBS (left panel) or vehicle alone (AOO) was applied (right panel) followed by challenge with Pd or DNFB. Data represent means ± SD of 10 ear samples and similar results were observed in two independent experiments. Asterisks (11) indicates statistical significance (<i>P</i><0.01) between Pd-challenged mice and DNFB-challenged mice. Lower panel shows combination of sensitization and challenge. (B) BALB/c nude mice were adoptively transferred with naïve SLN cells (open circle) or 8th Pd-SLN cells (filled square) as described. Seven days after the transfer, recipient mice (n = 5–8) were challenged with 10 nmol of Pd. The ear swelling was measured as described in(A). Data are represented as the mean ± SD. Asterisks (11) indicates statistical significance (<i>P</i><0.01). (C) Ear swelling at 24 hours after Pd challenge was compared between 1st, 4th, and 8th adoptive transfer of Pd-SLN cells. Data are represented as the mean ± SD. Asterisks (11) indicates statistical significance (<i>P</i><0.01).