Sox9 potentiates BMP2-induced glycosaminoglycans synthesis in MSCs in micromass cultures.

<p>(A): Real-time PCR for the expression of chondrogenic differentiation marker gene ACAN were conducted on day 7 and day 14 after infection of AdGFP, AdBMP2, and/or AdSox9, using GAPDH as a house keeping gene. (B–C): Alcian blue staining for sulfated glycosaminoglycans in micromass cultures of C3H10T1/2 cells on day 7 and day 14 after transduction of indicated recombinant adenoviruses, gross observation (B) and microscope examination (C, 40X) are shown. (D): Alcian blue staining quantifying: cells were extracted with 6 M guanidine hydrochloride, Optical density of the extracted dye was measured at 630 nm. The results were expressed as mean±SD of triplicate experiments, *<i>P<0.05</i>, ^#<i>P<0.01</i>.