Sox9 inhibits BMP2-induced osteogenic markers expression in MSCs in vitro.

<p>(A): Real-time PCR for the expression of early osteogenic differentiation gene Runx2 (Aa), late osteogenic gene osteocalcin (OC) and osteopontin (OPN) (b, c) were conducted at indicated time points after infection with AdGFP, AdBMP2, and/or AdSox9, using GAPDH as a house keeping gene. (B): Western blot for the expression of Runx2 (a), OPN (b) and OC (c) were conducted at indicated time points after transduction of indicated recombinant adenoviruses, respectively. (C) Relative protein expression was analyzed by quantity one software using β-actin as controls respectively. The results are expressed as mean±SD of triplicate experiments, *<i>P<0.05</i>, ^#<i>P<0.01</i>.