Dose-dependent effect of subditine (1) on cell membrane permeability, MMP and cytochrome c release.

<p>LNCaP and PC-3 cells were treated with subditine (<b>1</b>) for 24 h. Cells were then fixed and stained with membrane permeability dye, MMP, cytochrome c and Hoechst as described in Materials and Methods. (A) Stained cells were visualized using HSC arrayscan system to check nuclear morphology, membrane permeability, MMP integrity, cytochrome c release; Blue (nuclear), Green (Membrane permeability), Red (MMP), Cyan (cytochrome c release). (B) Bar chart showing the average fluorescent intensities of membrane permeability, MMP and cytochrome c (mean ± S.D.; *p<0.05).