Subditine (1) enhanced ROS production in LNCaP and PC-3.

<p>LNCaP and PC-3 cells were treated with subditine (<b>1</b>) (12.5 µM, 25 µM, 50 µM) for 24 h. Cells were then fixed and stained with DHE dye. ROS levels were indirectly determined by measuring DHE dye incorporation in the nuclear using Cellomic HCS arrayscan. Increased DHE dye intensity in the nucleus was detected upon treatment of subditine. Hoechst (blue) and DHE dye (green). Bar chart showing the average fluorescent intensity of DHE stain (mean ± S.D.; *p<0.05).