Inhibition of miR-184 reduces the proliferation of HCC cells.

<p><b>A</b>. Real-time PCR analysis of miR-184 expression in Hep3B and Huh7 cells transfected with miR-184 inhibitor. <b>B</b>. The proliferation ability of HCC cells transfected with an miR-184 inhibitor or negative control (NC), analyzed by the MTT assay. <b>C</b>. Representative micrographs (left) and quantifications (right) of crystal violet stained cell colonies formed by indicated HCC cell lines, 10 days after inoculation. <b>D</b>. The tumorigenicity of HCC cells transfected with miR-184 inhibitor or NC, as determined by an anchorage-independent growth ability assay. Colonies larger than 0.1 mm were scored. <b>E</b>. Flow cytometry analysis of indicated HCC cells transfected with miR-184-inhibitor or NC. Each bar represents the mean ± SD of three independent experiments. *<i>P</i><0.05.