Binding affinities of anti-α1 and β2 scFvs measured using fluorescence polarization.

<p>Purified scFv proteins were incubated with 6 or 4 µM (α1 or β2, respectively), of FITC-conjugated target peptide. Changes in polarization were measured in millipolarization (mP) units and plotted against concentration of the scFv. The K_d value of one representative trial is shown. Error bars correspond to standard deviations for duplicate measurements.