Comparison of detection limits of scFv and scFv-Fc.

<p>A. Binding specificity of equimolar concentrations of scFv-Fc and scFv A10, to different biotinylated peptides was detected in an ELISA using anti-Flag antibody conjugated to HRP. Signal intensities were measured at 405 nm and error bars indicate standard deviation of duplicate trials. NA, NeutrAvidin™; negative control pep, retinal protein Pep 1 peptide; beta 2, GABA_A receptor subunit β2 peptide; rho 1, GABA_c receptor subunit ρ pep tide and alpha 1, GABA_A receptor subunit α1 peptide. B. Dot blots of varying concentrations of MBP-α1 proteins treated with 10 nM A10 scFv-Fc (top row) and scFv (bottom row). Circles around signal spots indicate pencil markings of protein addition sites. C. Signal intensities normalized to highest signal obtained for each trail are plotted against log of concentration for scFv-Fc and scFv. Error bars depict standard deviations. n = 3.