Dissipation of the inner mitochondrial membrane potential (ΔΨ_m).

<p><b>(A)</b> Fluorescence microscopy of MCF-7 cells stained with the JC-1 probe after 72 h incubations of MCF-7 cells with combinations of NHAPI or its Fe complex with TMX. The scale bars represent 100 µm. Phase-contrast photomicrographs showing morphology of the same cells are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088754#pone.0088754.s011" target="_blank">Fig. S11</a>. <b>(B, C)</b> Flow cytometric analyses of MCF-7 cells treated with combinations of NHAPI or its Fe complex with TMX were stained with the JC-1 probe. Results in <b>(A)</b> are typical of <i>n</i>≥4 experiments; <b>(B, C)</b> Mean±SD (<i>n</i>≥4 experiments). Statistical significance (ANOVA): *<i>p<</i>0.05, **<i>p<</i>0.01, ***<i>p<</i>0.001 as compared to the control (untreated) group.