MEHP induced the mitochondrial membrane potential (MMP) loss in HUVEC cells.

<p>In MEHP treatment group, the HUVEC cells were treated with 0 µM, 25 µM, 50 µM and 100 µM MEHP for 24 hour. In NAC+MEHP treatment group, the HUVEC cells were pretreated for 1 hour before the MEHP treatment and then treated with 0 µM and 100 µM MEHP for 24 hour. After cultured with JC-1 for 30 minute at 37°C in the dark, the HUVEC cells in both groups were photographed by a fluorescence microscope (100X).Red fluorescence (A) represents mitochondria with intact membrane potential.Green fluorescence (B) represents de-energized mitochondria. The ratio of red fluorescence to green fluorescence was quantified, presented as mean± SEM; n = 3, * P<0.05 was considered as statistically significant difference compared to control group.(C).