MEHP attenuated the viability of HUVEC cells.

<p>In MEHP treatment group, the HUVEC cells were treated with MEHP (0, 6.25, 12.5, 25, 50 and 100 µM) for 24 and 48 hours. In NAC+MEHP treatment group, the NAC was dissolved in DMSO, the HUVEC cells was treated with NAC for 1 hour before MEHP treatment, and then the cells were treated with MEHP (0,100 µM) for 24 hours. The cell viability of both groups were measured by cell counting kit-8 (CCK-8). The treatment to control ratio of optic density represents the cell survival. Data from three independent experiment was represented in the form of mean±SEM; n = 6. * P<0.05 was considered as statistically significant difference compared to the control group. (B) MEHP induced cell apoptosis in HUVEC cells. In MEHP treatment group, the HUVEC cells were treated with MEHP (0, 6.25, 12.5, 25, 50 and 100 µM) for 24 hours. In NAC+MEHP treatment group, the HUVEC cells was treated with NAC for 1 hour before MEHP treatment, and then the cells were treated with MEHP (0,100 µM) for 24 hours. The cell apoptosis rate in both groups was measured by sub-G1 analysis. Data from three independent experiment was presented in the form of mean±SEM; n = 6. * P<0.05 was considered as statistically significant difference compared to control group.