Analysis of HuDe fibroblasts transfected with H-RasV12, H-RasV12S35, H-RasV12G37, H-RasV12C40 mutants.

<p>A, Immunoblotting analysis. Cell extracts were incubated with an anti-H-Ras antibody. As internal control an anti-β actin antibody was used. The increased expression of H-Ras with respect to empty vector transfected cells is shown. B, Growth curve. Cells were counted after the end of the selection with blasticidin-S (4 µg/ml) and cell number is reported. C, Cells morphology was examined by light microscopy, 200× total magnification. Arrows indicate flattened cells and cytosolic vacuolization associated with senescence.