Oncogenic versions of Ras promote a CD24⁺ to CD24⁻ transition and EMT in MCF10A and HMLE cell lines.

<p>(A) FACS analysis of CD24 and CD44 markers in MCF10A cells infected either with a K-Ras^V12 retroviral construct or the corresponding empty vector as a control. (B) Cell morphology of MCF10A cells infected either with a K-Ras^V12 retroviral construct or the corresponding empty vector as a control (upper panel) and HMLE infected either with a H-Ras^V12 retroviral construct or the corresponding empty vector as a control (lower panel). Images shown at 40x magnification. (C) CD24⁺ and CD24⁻ display epithelial and mesenchymal features, respectively. Top: Cell morphology. Bottom: Expression analysis as assessed by western-blotting of epithelial (E-cadherin, β-catenin) and mesenchymal (vimentin, fibronectin) markers in two independent clones for both cell populations. Images shown at 40x magnification.