<p>Log2-tranformed fold-changes comparing RT-qPCR and RNA-seq data normalized using RPM, TMM and BSN for transcripts increasing pre-ZGA (a), decreasing pre-ZGA (b), decreasing post-ZGA (c) and increasing post-ZGA (d).</p
International audienceBACKGROUND: Reverse transcription-quantitative polymerase chain reaction (RT-q...
International audienceIn the past 5 years, RNA-Seq has become a powerful tool in transcriptome analy...
International audienceBACKGROUND: Reverse transcription-quantitative polymerase chain reaction (RT-q...
<p>Shown are standard boxplots of normalized gene expressions. On the x-axis are the different sampl...
<p>Bar plots and inset scatter plot show a comparison of RNA-seq and RT-qPCR fold change values obta...
a<p>values based on RPKM normalization;</p>b<p>one qRT-PCR probe used;</p>c<p>not significant.</p
<p>Fold changes were calculated for 42 DEGs and a high correlation (R<sup>2</sup> > 0.93) was observ...
(a) Observed log2 fold change for spike-in constructed true positives plotted against the expected; ...
<p>Efficiencies of the primers: 9,718 = 1.9; 9,603 = 1.92; 6,367 = 2.07; 9,076 = 1.95; 5,222 = 2.16;...
<p>Efficiencies of the primers: 9,718 = 1.9; 9,603 = 1.92; 6,367 = 2.07; 9,076 = 1.95; 5,222 = 2.16;...
<p>Fold changes are expressed as the ratio of gene expression between XGC and YRC after normalizatio...
Different contrasts were marked in different colors. Different groups in the comparisons were labele...
Histograms of log2 of read counts by study indicate improved distribution overlap following normaliz...
<p>For each gene, 3 comparisons are shown in the figure. Upper panel: fold changes between RNA-Seq R...
Normalization of RNA-Seq data has proven essential to ensure accurate inferences and replication of ...
International audienceBACKGROUND: Reverse transcription-quantitative polymerase chain reaction (RT-q...
International audienceIn the past 5 years, RNA-Seq has become a powerful tool in transcriptome analy...
International audienceBACKGROUND: Reverse transcription-quantitative polymerase chain reaction (RT-q...
<p>Shown are standard boxplots of normalized gene expressions. On the x-axis are the different sampl...
<p>Bar plots and inset scatter plot show a comparison of RNA-seq and RT-qPCR fold change values obta...
a<p>values based on RPKM normalization;</p>b<p>one qRT-PCR probe used;</p>c<p>not significant.</p
<p>Fold changes were calculated for 42 DEGs and a high correlation (R<sup>2</sup> > 0.93) was observ...
(a) Observed log2 fold change for spike-in constructed true positives plotted against the expected; ...
<p>Efficiencies of the primers: 9,718 = 1.9; 9,603 = 1.92; 6,367 = 2.07; 9,076 = 1.95; 5,222 = 2.16;...
<p>Efficiencies of the primers: 9,718 = 1.9; 9,603 = 1.92; 6,367 = 2.07; 9,076 = 1.95; 5,222 = 2.16;...
<p>Fold changes are expressed as the ratio of gene expression between XGC and YRC after normalizatio...
Different contrasts were marked in different colors. Different groups in the comparisons were labele...
Histograms of log2 of read counts by study indicate improved distribution overlap following normaliz...
<p>For each gene, 3 comparisons are shown in the figure. Upper panel: fold changes between RNA-Seq R...
Normalization of RNA-Seq data has proven essential to ensure accurate inferences and replication of ...
International audienceBACKGROUND: Reverse transcription-quantitative polymerase chain reaction (RT-q...
International audienceIn the past 5 years, RNA-Seq has become a powerful tool in transcriptome analy...
International audienceBACKGROUND: Reverse transcription-quantitative polymerase chain reaction (RT-q...
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