ADAR1-p110 is involved in the editing of miR-376a.

<p>(a) The rate of miR-376a(e) in HFF cells either mock treated or HCMV infected was determined by next generation small RNA deep sequencing. Data are combined of two independent experiments. The rate in mock treated cells: 0.5943, in HCMV infected cells: 0.7912, *P-value: 4.38E-13. The rate of miR-376a(e) was calculated as the proportion of miR-376a(e) out of all the miR-376a species after allowing two mismatches. Hence no specific normalization was required. (b and c) WB analysis (b) and quantification (c) of ADAR1-p110 expression in mock treated or HCMV infected HFF cells transduced either with shADAR1 vector or with a control vector. (b) Data are representative of three independent experiments. Numbers indicate fold change in intensity compared to the expression of ADAR1-p110 observed after mock infection (M) in the control vector transduced cells. (c) Quantification of all experiments performed in (b). Shown are the relative average intensities ± S.D., *<i>P</i><0.001 by Student's t-test. (d) The rate of miR-376a(e) in HFF cells transduced either with a control vector (Ctrl) or shADAR1, and HCMV infected was determined by next generation small RNA deep sequencing. Data are combined of two independent experiments. The rate in control cells: 0.2806, in shADAR1 cells: 0.0.1461, *P-value: 1.6E-4. The rate of miR-376a(e) was calculated as the proportion of miR-376a(e) out of all the miR-376a species after allowing two mismatches. Hence no specific normalization was required. (e and f) WB (e) and quantification (f) of ADAR1-p110 expression in ADAR1-p110-transduced HFF cells. Data are representative of three independent experiments. Numbers indicate fold change in intensity of ADAR1-p110 compared to HFF cells transduced with an empty vector. (f) Shown are the relative average intensities of ADAR1-p110±S.D., *<i>P</i><0.002 by Student's t-test. (g) The rate of miR-376a(e) in HFF cells transduced either with a control vector or ADAR1-p110 was determined by next generation small RNA deep sequencing. Data are combined of two independent experiments. The rate in control cells: 0.3517, in ADAR1-p110 cells: 0.7653, *P-value: 3.97E-50. The rate of miR-376a(e) was calculated as the proportion of miR-376a(e) out of all the miR-376a species after allowing two mismatches. Hence no specific normalization was required.