Localization of gE-gI, gD and IgG under ABB-permissive and non-permissive conditions.

<p>HeLa cells transiently expressing gE-gI and gD-Dendra2 were incubated for 60 min at 37°C and 5% CO₂ with labeled IgGs (blue) and then fixed and processed for immunofluorescence using antibodies against gE (red) and gD-Cerulean (green). (A) Representative confocal slices (from three independent experiments in which ≥30 cells were analyzed) from cells treated with anti-gD_hFc (top panel), IgG_hFc (middle panel), or anti-gD_mFc (bottom panel). Regions of gE-gD colocalization appear yellow; regions of gD-IgG colocalization appear cyan, regions of gE-IgG colocalization appear magenta, and regions of triple colocalization appear white. Scale bar = 10 µm. (B) 3-D thresholded Pearson correlation coefficient analyses for data from ≥30 cells. Correlation coefficients are presented as the mean and standard deviation from experiments repeated at least three times. Asterisks (*) indicate a significant difference of colocalization compared to other members in the same category (p value<0.01).