Analysis of GPER localization by conventional and confocal immunofluorescence microscopy.

<p>Representative images of two independent experiments each performed in duplicates. A: Immunofluorescence microscopy showing a different expression pattern in MCF7 (predominantly nuclear) and T47D (predominantly cytoplasmic). The GPER-negative MDA-MB231 cell line was used as negative control. B: Confocal microscopy in T47D and MCF7 using a Leica SP5 microscope (with Leica HyD hybrid detection system). T47D show a strong GPER expression which is mainly localized in the cytoplasm of the cell. No distinct membranous expression was observed. MCF7 show a less strong GPER expression, which is clearly detectable inside the nucleus by analysis of the confocal images.