PABA reduces H₂O₂ accumulation in heat stressed strains <i>02</i> and <i>8213</i>.

<p>(A) Time course of accumulation of H₂O₂ in the mycelia of strains <i>02</i>, <i>8213</i>, <i>TB-2</i> and <i>TB-3</i> under heat stress (33°C). Strains <i>02</i>, <i>TB-2</i> and <i>TB-3</i>, which produce more PABA content than strain <i>8213</i>, have less H₂O₂ accumulation under parallel conditions. (B) Effects of PABA and PABA synthase inhibitor sulfanilamide on H₂O₂ accumulation. Strain <i>02</i> and strain <i>8213</i> were cultured on PDA medium with 1 mM PABA or 0.1 mM sulfanilamide for 6 days, then were subjected to high temperature treatment (33°C) for 48 hours as indicated, followed by H₂O₂ content measurement. Three independent biological replicates were performed for each analysis. Data are expressed as average ± SEM. Unpaired t-tests were performed between control sample and sulfanilamide treated sample or PABA treated sample within each strain, ns: P>0.05, *: P<0.05, **: P<0.01.