Prussian Blue staining close to the injection site of the rat shown in Figure 8 in a vehicle control rat.

<p>(<b>A</b>) Slice fluorescence levels are generally low in the cryo slices and in most cases restricted to vasculature as shown here for some ependymal cells of the chorioid plexus. (<b>B</b>) shows the Prussian Blue staining, whereas in healthy rats iron accumulations (from micro bleedings) in the brain are typically absent (<b>C</b>) is a maximum difference projection of (<b>A</b>) and (<b>B</b>). (<b>D</b>) The slice fluorescence vanishes during Prussian Blue staining and brightfield imaging. Abbreviations: F = fluorescence; LV = lateral ventricle; CC = corpus callosum. Magnification = 20x.