Computerized analysis of cytoplasmic Prussian blue (PB) stained AM.

<p>Analysis of iron deposits in AM and cell size as a surrogate parameter for cell activation of AM obtained from female wild-type and <i>Hfe^−/−</i> mice and <i>Hfe^LysMCre</i> mice at 4 h after intratracheal instillation of vehicle or 20 µg LPS. (A) PB-stained iron deposits in AM of female wild-type and <i>Hfe^−/−</i> mice. ^‡<i>P</i><0.05 and ^★<i>P</i>≤0.001 versus WT control mice; ^†<i>P</i><0.005 versus <i>Hfe^−/−</i> control mice. (B) AM size in female wild-type and <i>Hfe^−/−</i> mice. n = 5–7 per group. ^★<i>P</i>≤0.001 versus WT control mice; ^†<i>P</i><0.005 versus <i>Hfe^−/−</i> control mice. (C) PB-stained iron deposits in AM of <i>Hfe^LysMCre</i> mice. ^★<i>P</i>≤0.001 versus <i>Hfe^LysMCre</i> (−) control mice; ^†<i>P</i><0.001 versus <i>Hfe^LysMCre</i> (+) control mice. (D) AM size in <i>Hfe^LysMCre</i> mice. n = 4–15 per group. ^†<i>P</i><0.05 versus <i>Hfe^LysMCre</i> (+) control mice.