Proteomic comparison of miR-34a and miR-34c targets.

<p>(<b>A</b>) The correlation of log2 fold changes between miR-34a and miR-34c in the same transfection experiment (n = 2419) show a lower Spearman correlation than the two replicates of miR-34a (n = 1404) (<b>B</b>). This holds also true when comparing miR-34a experiments from different days (n = 1777) (<b>C</b>). Spearman coefficients for all proteins are marked in black, while seed containing proteins are indicated in red. (<b>D</b>) The overlap of common targets between miR-34a and miR-34c is rather low. (<b>E</b>) The overlap of miR-34a targets (–0.3 log2 FC) from SW480 cells <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092166#pone.0092166-Kaller1" target="_blank">[29]</a> is bigger with miR-34a than with miR-34c targets in our HeLa dataset. Venn diagrams show the overlap of the 81 down-regulated proteins quantified in both the Sw480 and our HeLa dataset. Numbers in Venn diagrams depict total number of proteins down-regulated by log2 < –0.3 for one miR-34 or shared by two miR-34 members. The percentage of down regulated SW480 proteins that are also down regulated in HeLa cells is given above the diagrams. (<b>F</b>) The overlap with miR-34a targets in SW480 cells is more significant for miR-34a than for miR-34c in HeLa cells (hypergeometric test).