Comparison of 5′-reference miRNA and 5′-shifted isomiR expression levels among MIN6 cells, human beta cells, and human islet.

<p>(<b>A</b>) The x-axis lists selected 5′-reference miRNAs in MIN6 (red), human beta cells (green), and human islets (blue). The y-axis depicts the Log10 of the average read count per million for each 5′-reference miRNA in each sample. (<b>B</b>) The x-axis shows the highly expressed 5′-shifted isomiRs ordered from left to right by decreasing fold-difference between primary human beta cells and MIN6 cells. The y-axis depicts the average read count per million for each 5′-shifted isomiR. (<b>C</b>) The number of genes with at least one conserved target site for miR-375 (gray), miR-375+1 (green), and miR-375-1 (orange) is shown. All sets are mutually exclusive: for example, a total of 390 genes have predicted conserved miR-375 target sites (42 unique to miR-375, 3 shared with miR-375+1 only, 337 shared with miR-375-1 only, and 8 common to all three).