Immunocytochemical detection of MAP2, GFAP and SOX14 in undifferentiated P19 and differentiated P19-N cells.

<p>Panel I: Immunocytochemical detection of MAP2 and GFAP-positive cells in P19-N. Panel II: Immunocytochemical detection of MAP2 and SOX14-positive cells in P19 and P19-N. The P19-N population consists of a large number of MAP2 terminally differentiated neurons (C and D), and a few GFAP-positive astroglial cells (B and D). Specific SOX14 immunoreactivity/punctated nuclear signal was detected in a majority of cells in differentiated P19-N cultures (I, K and M), and at basal level in P19 cells (F). DIC transmitted light images show morphology of SOX14+ cells in P19-N population (G and H). Yellow arrowhead in G-I marks flat cells with large nuclei which show strong SOX14 immunoreactivity. SOX14 is expressed in MAP2-positive neurons (K, arrows in L and M) and in non-neuronal cells (K, arrowheads in L and M). Boxed regions in J and K are enlarged in the same figures. Cell nuclei were counterstained with DAPI (A, D, E, H, J and L). Scale bars: A–K 50 μm, L and M 20 μm.