BDNF release from spiral ganglion cells is increased after co-treatment with Rolipram and BDNF.

<p>The concentration of released BDNF from spiral ganglion cells after treatment with Rolipram (R; 48 h and 30 min), BDNF (48 h) or both substances (BDNF 47R 30 min) was measured by ELISA. The supernatant of cells treated with BDNF alone or in combination with Rolipram contained significant higher concentrations of BDNF in comparison to supernatants from untreated cultures or from cells treated only with Rolipram. Application of Rolipram for 30 min to BDNF pre-treated cells resulted in a strong increase of BDNF in the supernatant. All values are given as mean ± SEM. N = 3; n = 3 except for the untreated and Rolipram 48 h group: N = 2, n = 3; One-way ANOVA with Tukey's multiple post hoc test was used to compare means: *p = 0.05; **p = 0.01; ***p = 0.001. Reference of the significance is marked by the thick bar. N gives the number of independent approaches; n gives the number of samples per approach.